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The church of St. Ambrose was built on the site of Florence where St. Ambrose is said to have stayed in 393. The church is first documented in 998, but it is probably older. The present church was rebuilt by Giovanni Battista Foggini in the 17th century. The church contains numerous frescoes, altarpieces and other works of art attributed to Andrea Orcagna, Agnolo Gaddi, Niccolò di Pietro Gerini, Lorenzo di Bicci, Masaccio, Fra Filippo Lippi, Sandro Botticelli, Alesso Baldovinetti and Fra Bartolomeo.

The present church of Sant'Ambrogio was built in 1590 and is located on top of an older church dating from the mid-13th century. The church was enlarged in 1740 when it took on its present appearance. The last restoration of the church was in 1992.

The church of Sant'Andrea was built between 1532 and 1701 on an ancient medieval church belonging to the Della Marra family. In 1532 the church was sold to the Order of Friars Minor, who undertook its reconstruction. With the edict of Murat in 1809, which provided for the suppression of religious orders, monastic life ceased in the church's convent. It was therefore handed over to the municipality, while the church remained open for liturgical celebrations.

The church of Sant'Andrea was built in the 1840s with the adjoining presbytery according to the classical design of the engineer Michele Cervelli from Lucca.

The church of Sant'Anna alle Paludi dates back to the 14th century when it was built by the University of Hortolani, although very few traces of the original structure remain, mainly due to the renovations of the 18th-19th and early 20th centuries. The church has a single nave designed according to the original 18th-century plan. The simple, linear lines only become beautiful neo-baroque in the decoration of the dome, which remains the only completed part of the church, according to the design of the architect Alfredo Belli from the late 19th century.

The church of Sant'Anna dei Pompetti, together with the neighbouring church of Santa Caterina, was originally part of the complex of the ancient cathedral of Santa Maria Aprutiensis from the 6th century. The building is made up of what has survived and been preserved from the old cathedral building.

The church of Sant'Anna, with the adjoining convent, was founded in 1584 on the initiative of Father Nicolò di Gesù Maria Doria (1539-1594). Shortly after its foundation, a pharmacy was opened there, attracting many patients in need of treatment. The first historical documents relating to the pharmacy date back to 1650, when there is mention of an "apothecary shop" belonging to the Discalced Carmelites.

The church of Sant'Anna was founded in the middle of the 13th century with a monastery that soon passed to the Benedictines. The old church was in a state of ruin, and its complete reconstruction began on 4 October 1634, based on a project by Francesco Contini. The work was completed in 1659. During the second French occupation (1806-1814), following the decree of 28 July 1806, the church of St. Anne was abolished together with the convent. The church is still desecrated.

The church of Sant'Anselmo all'Aventino, despite its appearance, is a recent construction. It was built in a neo-Romanesque style by Francesco Vespignani between 1892 and 1896. The interior consists of a nave and two aisles divided by granite columns, a latticed ceiling and an apse decorated with mosaics; the crypt with five aisles is particularly large. The church is built on the remains of a Roman Domus of the 2nd-3rd century AD, which can be seen in the basement of the church.

The church of Sant'Antonino martire is first mentioned in the 12th century. It is believed that the church of Sant'Antonino was first rebuilt in the Venetian-Byzantine style between the 12th and 13th centuries. It then underwent various reconstructions that gave it a Gothic appearance. In the second half of the 17th century, it was completely rebuilt according to a project attributed to Baldassare Longhena, who was in fact the supervisor of the work. The bell tower was completed in 1750, apparently to a design by the parish priest of the time, Antonio Fusarini.